Hybrid tandem mass spectrometry of electrospray‐produced peptide ions

The collision‐induced decomposition spectra of electrospray‐produced molecule ions were investigated on a hybrid tandem mass spectrometer. The molecular mass of the peptides investigated ranged from m/z 409 (as the singly protonated molcule ion) to 1758 (as the singly, doubly and triply protonated molecules). The effect of changing the center‐of‐mass collision energy by changing either the laboratory collision energy or the mass of the target gas was investigated. The spectra of all ions investigated showed a large number of sequence ions representative of the peptides' structures.