Key result
TaqMan RT-qPCR achieves ~73% pre-clinical CSFV detection, outperforming conventional RT-PCR and Ag-ELISA.
Why the study?
Classical swine fever is a highly contagious, devastating viral disease, and rapid detection and identification of the causative agent is crucial to control infection in pigs.
Does TaqMan RT-qPCR improve the pre-clinical detection of Classical swine fever virus in pigs compared to conventional RT-PCR and Ag-ELISA?
Cross-Sectional (n=70)
Does TaqMan RT-qPCR improve the pre-clinical detection of Classical swine fever virus in pigs compared to conventional RT-PCR and Ag-ELISA?
Absolute Event Rate: 73.33% vs 60%
TaqMan-based RT-qPCR is more sensitive than conventional RT-PCR and Ag-ELISA for the early, pre-clinical detection of Classical swine fever virus in pigs.
May support higher sensitivity of TaqMan RT-qPCR for pre-clinical CSFV detection in pigs; leaves open applicability pending validation studies.
Classical swine fever (CSF) is a highly contagious and devastating viral disease, causing serious losses in the pig industry worldwide. Rapid detection and identification of the causative agent is a crucial step in controlling CSF infection in pig population. In the present study, a fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase real time PCR assay (RT-qPCR)was evaluated parallel to conventional RT-PCR and antigen capture ELISA to detect Classical swine fever virus (CSFV) in the pre-clinical phase of the disease. In addition, hematological analysis was performed at different clinical phases in order to diagnose CSF pre-clinically. Thrombocytopenia and leucopenia were early clinical clues recorded in CSFV infected pigs. Single step RT-qPCR confirmed the presence of CSFV nucleic acid in blood, nasal swabs, ocular swabs as well as in tonsillar scrapings in the pre-clinical phase. CSFV nucleic acid was detected with maximum positivity in blood and tonsillar scrapings (70-73%) using RT-qPCR as compared to 60% and 33.33-40% positivity in conventional RT-PCR and Ag-ELISA, respectively. Thus, TaqMan based RT-qPCR assay can be used as an efficient assay for rapid CSFV detection at pre-clinical phase of the disease to contain the disease from in-contact infected pigs to susceptible population.
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Bora et al. (2021) conducted a cross-sectional in Classical swine fever (CSF) (n=70). Single Step TaqMan Real-time PCR Assay (RT-qPCR) vs. Conventional nested RT-PCR and Antigen-Capture ELISA was evaluated on Detection of CSFV in whole blood during the pre-clinical phase. Single step TaqMan RT-qPCR detected Classical swine fever virus with maximum positivity (73.33%) in blood samples during the pre-clinical phase, compared to 60% by conventional RT-PCR and 33.33% by Ag-ELISA.
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