Barley grist which has been extracted by aqueous sodium chloride to remove free β-amylase liberates an additional amount of enzymic material on treatment with saline solutions of thioglycollic acid. The “latent” enzyme set free In this way has been concentrated and compared with free β-amylase, which it so resembles that substantial identity between the free and latent enzymes may be assumed.
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Pollock et al. (1958) studied this question.
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