What question did this study set out to answer?

To evaluate whether the peptide intermedin 1–53 prevents vascular smooth muscle cell calcification by suppressing endoplasmic reticulum stress via the cAMP/PKA pathway.

September 4, 2013Experimental Biology and Medicine

Intermedin 1–53 attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway

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Authors

JCJin-Rui ChangXi'an Medical University BZBaohong ZhangNational Instruments (Ireland)XTXu TengHebei Medical University

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Implication

Experimental study demonstrates that intermedin 1–53 reduces calcification in vascular smooth muscle cells, indicating a protective mechanism via endoplasmic reticulum stress inhibition.

Key Points

To evaluate whether the peptide intermedin 1–53 prevents vascular smooth muscle cell calcification by suppressing endoplasmic reticulum stress via the cAMP/PKA pathway.
Induced calcification in cultured vascular smooth muscle cells using calcium chloride and beta-glycerophosphate, and provoked endoplasmic reticulum stress using tunicamycin or dithiothreitol.
Treated cells with intermedin 1–53 (10⁻⁷ mol/L) with or without the protein kinase A inhibitor H89, assessing apoptosis, lineage markers, and mineral deposition using Alizarin Red S staining.
Intermedin 1–53 significantly reduced protein expression of key endoplasmic reticulum stress markers (ATF4, ATF6, GRP78, and GRP94) and suppressed both CHOP and cleaved caspase 12 levels.
Inhibition of protein kinase A with H89 completely abolished the protective and anti-stress effects exerted by intermedin 1–53.
Intermedin 1–53 restored vascular smooth muscle cell lineage markers while decreasing alkaline phosphatase activity and calcium nodule formation.

Cite This Study

Chang et al. (2013) studied this question.

synapsesocial.com/papers/6aa7aa414103b36f81189b93 https://doi.org/10.1177/1535370213502619

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Implication

Experimental study demonstrates that intermedin 1–53 reduces calcification in vascular smooth muscle cells, indicating a protective mechanism via endoplasmic reticulum stress inhibition.

Intermedin 1–53 attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

Intermedin 1–53 attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

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