Key result
Monolayer culture of embryonic cardiac myocytes decreases tissue-specific MHC reactivity, suggesting lost differential expression.
The intrinsic program of cardiac myogenesis may not be sufficient to maintain differential expression of tissue-specific myosin heavy chain isoforms in monolayer cell culture.
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Monolayer culture may fail to sustain myosin isoform expression in embryonic myocytes; leaves open microenvironmental requirements for maintaining differentiation in vitro.
Zadeh et al. (1986) studied this question. Monolayer cell culture vs. Before plating / in vivo was evaluated on Myosin heavy chain (MHC) content and isoform expression. Monolayer culture of chick embryonic cardiac myocytes resulted in decreased reactivity with tissue-specific myosin heavy chain antibodies by 7 days, suggesting loss of differential expression.
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