Key result
RT-PCR broadly matches ELISA but finds no FMD in ~23% of premises, suggesting over-reporting.
Population
Samples from 1,720 infected premises out of 2,030 designated cases of foot-and-mouth disease in the UK in 2001
Comparison
Retrospective analysis by real-time reverse… vs Original laboratory results
Design
Other
Authors
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May indicate FMD over-reporting; leaves open whether RT-PCR confirmation could refine future outbreak surveillance accuracy.
Observational (n=1,730)
Retrospective RT-PCR showed broad agreement with original ELISA/VI for FMD detection, but missed some variants, and suggested FMD incidence may have been over-reported by approximately 23%.
Ferris et al. (2006) conducted an observational in Foot-and-mouth disease (n=1,730). Real-time reverse transcriptase-PCR (RT-PCR) vs. ELISA and virus isolation was evaluated on Detection of FMD virus. Retrospective analysis by RT-PCR showed broad agreement with ELISA and virus isolation, though no evidence of FMD was found in 23% (390 of 1730) of sampled premises, suggesting over-reporting.
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