Application of a real‐time PCR method to detect Pyrenophora species in barley seed, and implications for seed treatment strategies*

DNA primer sequences and the polymerase chain reaction (PCR) were used to detect the presence of Pyrenophora graminea and P. teres on barley seeds. The PCR was carried out in a Lightcycler with real‐time measurement of the product using a fluorescent dye. P. teres infection was quantified, while P. graminea infection was determined as present or absent. Test results were available within 48 h. Seed treatments to control both species are commonly used in the UK, though recent results indicated that P. graminea occurs infrequently, while incidence of P. teres is more variable. Rapid seed‐health testing techniques offer the potential for improved targeting of seed treatment without delaying the processing of seed.