Microbial analysis uncovers novel chemolithoautotrophs in hot springs, indicating that thermal ecosystems harbor uncharacterized acetogenic bacterial lineages.
Key Points
To identify and isolate novel thermophilic acetogenic prokaryotes from geothermal hot spring microbial communities.
Analyzed microbial community profiles from five hot springs using molecular and classical microbiological techniques.
Cultivated stable enrichment cultures under chemolithoautotrophic growth conditions supplied with hydrogen (H2) and carbon dioxide (CO2).
Purified and isolated a targeted acetogenic bacterial strain into pure culture.
Obtained stable chemolithoautotrophic enrichment cultures containing Aceticella and an unclassified genus from the family Neomoorellaceae despite low natural environmental abundance.
Successfully isolated a pure culture representing a novel genus and species within the family Neomoorellaceae.