Evaluation of Zinc Selenide and Vanadium Selenide Nanomaterials for Wound Healing and Inflammatory Regulation in a Rabbit Model

In this study, zinc selenide (ZnSe) and vanadium selenide (V 2 Se 9 ) nanoparticles (NPs) were synthesized via a hydrothermal method and evaluated for their potential for wound healing using Oryctolagus cuniculus as a surgical wound model. Physicochemical characterization confirmed the nano‐structures with hydrodynamic diameters of 131 nm (ZnSe) and 136 nm (V 2 Se 9 ) and stable zeta potentials. ZnSe NPs showed excellent antibacterial response, with inhibition zones (21 ± 1 mm against Escherichia coli and 20 ± 1 mm for Staphylococcus aureus ) comparable to the standard drug, whereas V 2 Se 9 NPs exhibited mild activity. In vivo wound‐healing trials indicated that ZnSe NPs‐treated groups achieved the highest wound closure (93.6%) within 18 days, surpassing V 2 Se 9 (90.1%) and control groups. Histopathological investigations confirmed enhanced collagen deposition and epithelialization and reduced inflammatory infiltration in wounds treated with ZnSe NPs. Moreover, ZnSe NPs modulated pro‐inflammatory cytokine IL‐6 more effectively, lowering levels to 15.7 pg/mL by day 18. The results of this study demonstrate that ZnSe NPs have promising antibacterial, antioxidant, anti‐inflammatory, and wound healing properties. These findings support their potential as a strong candidate wound‐healing agent for rabbits, although further in vitro cytocompatibility and detailed biosafety studies are required before clinical translation.