Using High-Performance Liquid Chromatography for the Quantitation Analysis of Amino Acids in Vietnam Edible Bird’s Nest

This paper describes a method utilizing high-performance liquid chromatography with fluorescence detection (HPLC-FLD) developed for the simultaneous quantitation analysis of 15 acidic amino acids in Edible Bird’s Nest. Samples underwent hydrolysis in acidic media (1:1 HCl) at 100°C for 16.0 hours, followed by neutralization with NaOH (30%). The mean recoveries of the 15 analytes ranged from 80.1% to 110.8%, with MDL and MQL values of 2.0 ppm and 6.0 ppm, respectively. The method demonstrated accuracy, repeatability, and recovery unaffected by matrix effects. Its application enabled the determination of total amino acids content in Vietnam bird's nest samples, yielding results of 43.30 to 45.08 g/100 g for refined bird's nest, 36.69 to 41.40 g/100 g for raw bird's nest, and 1221 to 1723 mg/kg for distilled bird's nest.