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The aim of study was the production of polyclonal antibody of erythropoietin hormone (Anti-EPO) to determine EPO in human serum by liquid-phase radioimmunoassay (RIA). Production of anti-EPO was performed by immunizing Balb/C mice with EPO antigen using primary immunization and four booster doses. EPO tracer (125I-EPO) was prepared using Chloramine-T method. Additionally, EPO standards were mad. Furthermore, the assay was optimized and validated in additional studies. In conclusion, the elaborated EPO-RIA system exhibits a simple, accurate, specific and sensitive method for determining EPO concentration in human serum and might be suitable for clinical diagnosis of myeloproliferative diseases.
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Ebeid et al. (2023) studied this question.
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