Key result
Rhizoma Drynariae alters 149 genes in ovariectomized rats, targeting circadian rhythm and inflammatory pathways.
Why the study?
Osteoporosis impairs quality of life in older populations, and the differential gene expression and mechanisms of Rhizoma drynariae remained to be analyzed using transcriptome sequencing.
Does Rhizoma drynariae alter gene expression in ovariectomized rats with osteoporosis?
Does Rhizoma drynariae alter gene expression in ovariectomized rats with osteoporosis?
Effect estimate: 149 differentially expressed genes (44 downregulated, 105 upregulated)
p-value: p=<0.05
Rhizoma Drynariae alters the expression of 149 genes in ovariectomized rats, suggesting potential targets in circadian rhythm, lipid metabolism, and inflammatory pathways for osteoporosis treatment.
Hypothesis-generating in ovariectomized rats; leaves open translation to human osteoporosis therapy pending clinical validation.
Osteoporosis is increasingly becoming a serious problem affecting the quality of life of the older population. Several experimental studies have shown that Chinese medicine has a definite effect on improving osteoporosis. Based on transcriptome sequencing, we analyzed the differential gene expression and mechanism of the related signaling pathways. Fifteen rats were randomly divided into an experimental group, a model group, and a sham surgery group. The rat model for menopausal osteoporosis was established using an ovariectomy method. One week after modeling, the experimental group was administered(intragastric administration)8.1 g/kg of Rhizoma drynariae, whereas the model and sham groups received 0.9% saline solution twice daily for 12 weeks. Subsequently, the rats were sacrificed, and the left femur of each group was removed for computerized tomography testing, while right femurs were used for hematoxylin and eosin staining. High-throughput RNA sequencing and functional and pathway enrichment analyses were performed. Comparing the gene expression between the experimental and model groups, 149 differential genes were identified, of which 44 were downregulated and 105 were upregulated. The criteria for statistical significance were |log2 Fold Change| > 1 and P < 0.05. Gene ontology analysis showed that the differentially expressed genes were enriched in cell component terms such as cell part and outer cell membrane part, and the genes were associated with cell process, biological regulation, metabolic processes, DNA transcription, and catalytic activity. Enrichment analysis of Kyoto Encyclopedia of Genes and Genomes pathways showed significantly enriched pathways associated with systemic lupus erythematosus, herpes simplex infection, circadian rhythm, vascular smooth muscle contraction, the AGE-RAGE signaling pathway in diabetic complications, and the TNF, Apelin, and Ras signaling pathways. Our results revealed that the Npas2, Dbp, Rt1, Arntl, Grem2, H2bc9, LOC501233, Pla2g2c, Hpgd, Pde6c, and Dner genes, and the circadian rhythm, lipid metabolism, inflammatory signaling pathway, and immune pathways may be the key targets and pathways for traditional Chinese medicine therapy of Rhizoma Drynariae in osteoporosis.
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Su et al. (2022) studied Osteoporosis (n=15). Rhizoma Drynariae vs. 0.9% saline solution twice daily was evaluated on Differential gene expression between experimental and model groups (149 differentially expressed genes (44 downregulated, 105 upregulated), p=<0.05). Rhizoma Drynariae treatment in ovariectomized rats resulted in 149 differentially expressed genes compared to the model group, highlighting targets in circadian rhythm and inflammatory pathways.
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