What question did this study set out to answer?

To determine whether immunohistological profiling of cytotoxic molecules and regulatory T-cell markers can reliably differentiate early Stevens–Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) from erythema multiforme major (EMM).

March 28, 2012The Journal of DermatologyOpen Access

Distinguishing between erythema multiforme major and Stevens–Johnson syndrome/toxic epidermal necrolysis immunopathologically

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Authors

SIShinsaku IWAI HSHirohiko Sueki HWHideaki Watanabe

Discussion

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Overview

Comparative biopsy analysis demonstrates distinct cytotoxic and regulatory T-cell markers in SJS/TEN versus EMM, indicating utility for early differential diagnosis.

Key Points

To determine whether immunohistological profiling of cytotoxic molecules and regulatory T-cell markers can reliably differentiate early Stevens–Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) from erythema multiforme major (EMM).
Analyzed skin biopsy specimens collected over a 9-year period from 14 patients with SJS/TEN and 16 patients with EMM.
Performed double immunofluorescence labeling of CD8 with granulysin, perforin, or granzyme B, and immunohistochemical staining for CD1a, CD3, CD4, CD8, CD68, and Foxp3, quantifying positive cells across five high-power fields.
Proportions of granulysin(+) cells per CD8(+) cell (P = 0.012) and perforin(+) cells per CD8(+) cell (P = 0.037) were significantly higher in SJS/TEN lesions than in EMM lesions.
Numbers of Foxp3(+) cells (P = 0.004) and CD4(+) cells (P = 0.0017) per five high-power fields were significantly lower in SJS/TEN compared to EMM.

Cite This Study

IWAI et al. (2012) studied this question.

synapsesocial.com/papers/6aa93d5ff8b27f8ac662efdd https://doi.org/10.1111/j.1346-8138.2012.01532.x

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March 28, 2012The Journal of DermatologyOpen Access

Distinguishing between erythema multiforme major and Stevens–Johnson syndrome/toxic epidermal necrolysis immunopathologically

View Full Paper

Ask AI

Bookmark

Authors

SIShinsaku IWAI HSHirohiko Sueki HWHideaki Watanabe

Discussion

Member takes

Overview

Comparative biopsy analysis demonstrates distinct cytotoxic and regulatory T-cell markers in SJS/TEN versus EMM, indicating utility for early differential diagnosis.

Key Points

To determine whether immunohistological profiling of cytotoxic molecules and regulatory T-cell markers can reliably differentiate early Stevens–Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) from erythema multiforme major (EMM).
Analyzed skin biopsy specimens collected over a 9-year period from 14 patients with SJS/TEN and 16 patients with EMM.
Performed double immunofluorescence labeling of CD8 with granulysin, perforin, or granzyme B, and immunohistochemical staining for CD1a, CD3, CD4, CD8, CD68, and Foxp3, quantifying positive cells across five high-power fields.
Proportions of granulysin(+) cells per CD8(+) cell (P = 0.012) and perforin(+) cells per CD8(+) cell (P = 0.037) were significantly higher in SJS/TEN lesions than in EMM lesions.
Numbers of Foxp3(+) cells (P = 0.004) and CD4(+) cells (P = 0.0017) per five high-power fields were significantly lower in SJS/TEN compared to EMM.

Cite This Study

IWAI et al. (2012) studied this question.

synapsesocial.com/papers/6aa93d5ff8b27f8ac662efdd https://doi.org/10.1111/j.1346-8138.2012.01532.x

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