What question did this study set out to answer?

To evaluate the capacity of alpha-melanocyte-stimulating hormone and its COOH-terminal tripeptide fragment to inhibit endotoxin-induced tumor necrosis factor-alpha production in human astrocytic cells.

September 12, 1997NeuroImmunoModulation

A Potential Mechanism of Local Anti-Inflammatory Action of Alpha-Melanocyte-Stimulating Hormone within the Brain: Modulation of Tumor Necrosis Factor-Alpha Production by Human Astrocytic Cells

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Authors

KWKit Yue WongThe University of Texas Southwestern Medical Center NRNilum RajoraThe University of Texas Southwestern Medical Center GBG BoccoliUniversity of Milan

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Implication

Laboratory study demonstrates alpha-MSH inhibits endotoxin-induced TNF-alpha in human astrocytic cells, suggesting therapeutic potential for neuroinflammatory conditions.

Key Points

To evaluate the capacity of alpha-melanocyte-stimulating hormone and its COOH-terminal tripeptide fragment to inhibit endotoxin-induced tumor necrosis factor-alpha production in human astrocytic cells.
Cultured human glioma cells (A-172 anaplastic astrocytoma cell line) stimulated with bacterial endotoxin to induce TNF-alpha production.
Treated cells with alpha-MSH (1-13) or a COOH-terminal tripeptide fragment, testing both concurrent treatment and preincubation conditions.
Screened resting A-172 cells for human melanocortin-1 receptor mRNA expression.
Both alpha-MSH (1-13) and the COOH-terminal tripeptide fragment inhibited endotoxin-induced TNF-alpha production, with the full alpha-MSH (1-13) sequence demonstrating greater potency.
Preincubation of A-172 cells with alpha-MSH (1-13) markedly enhanced its inhibitory effect against TNF-alpha induction.
Melanocortin-1 receptor mRNA was identified in resting A-172 cells, pointing to a receptor-mediated mechanism for the observed anticytokine action.

Cite This Study

Wong et al. (1997) studied this question.

synapsesocial.com/papers/6aa93f19e6a6728514255d13 https://doi.org/10.1159/000097313

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Implication

Laboratory study demonstrates alpha-MSH inhibits endotoxin-induced TNF-alpha in human astrocytic cells, suggesting therapeutic potential for neuroinflammatory conditions.

A Potential Mechanism of Local Anti-Inflammatory Action of Alpha-Melanocyte-Stimulating Hormone within the Brain: Modulation of Tumor Necrosis Factor-Alpha Production by Human Astrocytic Cells

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Cite This Study

A Potential Mechanism of Local Anti-Inflammatory Action of Alpha-Melanocyte-Stimulating Hormone within the Brain: Modulation of Tumor Necrosis Factor-Alpha Production by Human Astrocytic Cells

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