In the course of the continuation of studies on the in vitro metabolism of dehydroepiandrosterone (I) by adrenal tissue, it was noted in paper chromatographic analyses of the incubated material that several substances of a more polar nature than 1 1β-hydroxy-Δ4-androstene-3,17-dione (IV) were being formed. Compound IV had been isolated and identified earlier after perfusion of I through cow adrenals (Meyer et al., 1953 a). Initial experiments with various tissue preparations, whole glands, bovine adrenal whole homogenates, and washed homogenate residues (Hayano & Dorfman, 1953), all yielded crude extracts which produced a similar papergram pattern of products in that polar region. Further incubations with the washed residue preparations showed that the addition of adenosine triphosphate (ATP) plus diphosphopyridine nucleotide (DPN) increased the conversion appreciably. Thus, with this supplementation a large scale incubation was carried out for isolation purposes.
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Meyer et al. (1955) studied this question.