Key result
Carbachol shows no effect on TPMP distribution or electrophysiological parameters in neuroblastoma cells.
Why the study?
Accurate estimation of membrane potential in neuroblastoma N1E 115 cells using [3H]triphenylmethylphosphonium cation requires evaluation under controlled conditions with suitable lipophilic anions.
The study evaluates the methodological use of [3H]TPMP for estimating membrane potential in neuroblastoma cells, noting limitations with specific lipophilic anions.
Answers are grounded in this paper and cite their sources.
No effect on [3H]TPMP in N1E-115 cells warrants caution for its use in muscarinic membrane potential assays; leaves open validation in other models.
The lipophilic permeant cation [3H]triphenylmethylphosphonium (TPMP) was used to estimate membrane potential in neuroblastoma N1E 115 cells under carefully controlled conditions. The cation distributes into the cells only in the presence of a lipophilic anion, and tetraphenylboron and picrate have been used for this purpose. The potassium salt of tetraphenylboron is poorly soluble, so that studies in high [K+] media are difficult with this anion whereas picrate, at the concentrations required, hyperpolarises the cells. The effect of muscarinic receptor activation was investigated by treating cells with carbachol but no effect was seen either on [3H] TPMP distribution or electrophysiological parameters. The use of [3H]TPMP for qualitative and quantitative evaluation of membrane potential in these cells is discussed.
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Milligan et al. (1984) studied Neuroblastoma. [3H]triphenylmethylphosphonium (TPMP) and carbachol was evaluated on Membrane potential and [3H] TPMP distribution. Muscarinic receptor activation with carbachol had no effect on [3H]TPMP distribution or electrophysiological parameters in neuroblastoma N1E 115 cells.
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