Key result
Grape seed extracts inhibit platelet aggregation in mice via tyrosine phosphatase inhibition without increasing bleeding.
Why the study?
Grape seed extracts reportedly inhibit platelet aggregation, but the underlying mechanism has not been elucidated.
Do grape seed extracts inhibit platelet aggregation in preclinical models?
Do grape seed extracts inhibit platelet aggregation in preclinical models?
Grape seed extracts inhibit platelet aggregation by inhibiting tyrosine phosphatase activity without increasing bleeding risk in mice, suggesting a potential mechanistic basis for the cardiovascular benefits of grape components.
Should not alter clinical antiplatelet practice; hypothesis-generating for grape seed extract as antithrombotic agent.
Platelets play an important role in various thrombotic diseases, including myocardial infarction. Because red wine consumption is inversely associated with death due to ischemic heart diseases, the effects of grape components on platelet function have been extensively investigated. Grape seed extracts (GSEs) reportedly inhibit platelet aggregation; however, the underlying mechanism has not been elucidated. We discovered that GSEs inhibit platelet aggregation induced by collagen and thrombin-receptor agonist peptide and increase basal levels of tyrosine phosphorylation, which was also observed in the presence of a protein tyrosine phosphatase (PTP) inhibitor. An in vitro phosphatase assay indicated that GSE dose dependently inhibited PTP-1B and Src homology 2 domain-containing phosphatase-1 activity, which positively regulates platelet aggregation. We propose that GSEs inhibit platelet aggregation by inhibiting tyrosine phosphatase activity. Moreover, we showed that GSE ingestion inhibited platelet aggregation in mice without enhancing tail bleeding, implying that GSE supplementation might be beneficial to prevention of thrombotic diseases.
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Jin et al. (2013) studied Thrombotic diseases. Grape seed extracts (GSEs) was evaluated on Platelet aggregation and tyrosine phosphatase activity. Grape seed extracts inhibited platelet aggregation in vitro and in mice by inhibiting protein tyrosine phosphatase activity, without enhancing tail bleeding.
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