What question did this study set out to answer?

To identify long non-coding RNAs necessary for oncogene-induced senescence and determine their regulatory mechanism in human fibroblasts.

January 29, 2018Nucleic Acids ResearchOpen Access

LncRNA-OIS1 regulates DPP4 activation to modulate senescence induced by RAS

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To identify long non-coding RNAs necessary for oncogene-induced senescence and determine their regulatory mechanism in human fibroblasts.
Analyzed differential lncRNA expression profiles comparing senescent and non-senescent human fibroblast cells.
Conducted an RNA interference loss-of-function screen targeting differentially expressed lncRNAs to detect senescence bypass.
Evaluated subcellular localization, cell proliferation, cell-cycle markers (CDKN1A), and downstream target interactions using knockdown and ectopic expression assays.
Knockdown of lncRNA-OIS1 facilitated senescence bypass, enhanced cell proliferation, decreased CDKN1A expression, and elevated cell-cycle-related gene expression.
Silencing lncRNA-OIS1 blocked the senescence-associated induction of the tumor suppressor DPP4.
Depletion of DPP4 replicated senescence bypass, whereas ectopic expression of DPP4 in lncRNA-OIS1-depleted cells rescued the senescent state.

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Implication

In vitro functional screen reveals lncRNA-OIS1 is required for oncogene-induced senescence via DPP4 activation in human fibroblasts, indicating a key tumor-suppressive role.

Cite This Study

Li et al. (2018) studied this question.

synapsesocial.com/papers/6aaa46e335c9841531c1fbec https://doi.org/10.1093/nar/gky087

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Implication

In vitro functional screen reveals lncRNA-OIS1 is required for oncogene-induced senescence via DPP4 activation in human fibroblasts, indicating a key tumor-suppressive role.

LncRNA-OIS1 regulates DPP4 activation to modulate senescence induced by RAS

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Discussion

Implication

Cite This Study

LncRNA-OIS1 regulates DPP4 activation to modulate senescence induced by RAS

Key Points

Discussion

Implication

Cite This Study

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