COL1A1 and COL3A1 elevation and regulation by β2-microglobulin in subsynovial connective tissues of patients undergoing hemodialysis with carpal tunnel syndrome

Kyoko Muneshige; Kenji Onuma; Yuji Yokozeki; Koji Sukegawa; Yuya Otake; Yukie Metoki; Mitsufumi Nakawaki; Makoto Itakura; Gen Inoue; Masashi Takaso; Kentaro Uchida

doi:10.21203/rs.3.rs-1872629/v1

July 26, 2022Open Access

COL1A1 and COL3A1 elevation and regulation by β2-microglobulin in subsynovial connective tissues of patients undergoing hemodialysis with carpal tunnel syndrome

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Key Points

To investigate collagen gene expression in the subsynovial connective tissue of hemodialysis patients with carpal tunnel syndrome and determine the regulatory role of β2-microglobulin.
Harvested subsynovial connective tissue specimens from 64 patients with carpal tunnel syndrome (17 receiving hemodialysis and 47 non-hemodialysis controls) during carpal tunnel release surgery.
Quantified COL1A1 and COL3A1 expression in tissue samples using quantitative polymerase chain reaction (qPCR).
Stimulated isolated subsynovial connective tissue cells in vitro with vehicle or β2-microglobulin (1 or 10 µg/ml) for 6 and 24 hours to evaluate collagen gene transcription.
Expression levels of COL1A1 and COL3A1 were significantly increased in the hemodialysis cohort compared to the non-hemodialysis cohort (P=0.021 for COL1A1; P=0.031 for COL3A1).
Stimulating subsynovial connective tissue cells with β2-microglobulin for 6 and 24 hours significantly elevated COL3A1 mRNA expression relative to vehicle controls (P<0.001).

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Implication

Comparative tissue analysis reveals elevated COL1A1 and COL3A1 driven by beta-2 microglobulin in hemodialysis carpal tunnel syndrome, suggesting a molecular pathway for fibrosis.

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Muneshige et al. (2022) studied this question.

synapsesocial.com/papers/6aaa50f8c0d26b63ae086495 https://doi.org/10.21203/rs.3.rs-1872629/v1

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COL1A1 and COL3A1 elevation and regulation by β2-microglobulin in subsynovial connective tissues of patients undergoing hemodialysis with carpal tunnel syndrome

Key Points

No abstract yet

Implication

Discussion

Cite This Study

COL1A1 and COL3A1 elevation and regulation by β2-microglobulin in subsynovial connective tissues of patients undergoing hemodialysis with carpal tunnel syndrome

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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