Key result
Indirect immunoperoxidase assay detects DSHDV antigens in infected duck tissues at ~4 hours postinoculation.
Why the study?
An improved indirect immunoperoxidase assay was needed for sensitive and specific detection of Duck swollen head hemorrhagic disease virus antigen in Pekin ducks.
The developed indirect immunoperoxidase assay provides a sensitive and specific method for the early diagnosis and study of DSHDV infection in ducks.
Hypothesis-generating for early DSHDV detection in ducks; prospective validation required before clinical adoption.
An improved indirect immunoperoxidase assay (IPA) was developed to detect antigens of Duck swollen head hemorrhagic disease virus (DSHDV) in paraformaldehyde-fixed, paraffin-embedded tissues of Pekin ducks (Anas platyrhynchos). This technique used an indirect streptavidin-alkaline phosphatase labeling system with polyclonal antiserum developed against purified DSHDV antigens. Specimens from the experimentally inoculated Pekin ducks with DSHDV and archived paraffin-embedded tissues from natural cases of Duck viral swollen head hemorrhagic disease (DVSHD) were examined by clinical and histological criteria. Positive staining was most widely observed in the cytoplasm of the following organs: immune, digestive, and urinary organs, heart, lung, and trachea, which corresponded to the intracellular distribution of reovirus. The DSHDV antigens were first detected at 4 hr postinoculation in the bursa of Fabricius of infected ducks. Therefore, this method was suitable for the early diagnosis of DVSHD. Immunoperoxidase staining was not present in tissues and organs of sham-inoculated ducks (negative control). The IPA developed in the current study is a convenient, sensitive, and specific means of detecting DSHDV and is applicable to routine diagnosis, retrospective studies, and prospective studies of DSHDV infection in ducks.
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Li et al. (2010) studied Duck swollen head hemorrhagic disease virus (DSHDV) infection. Indirect immunoperoxidase assay (IPA) vs. Sham-inoculated ducks (negative control) was evaluated on Detection of DSHDV antigens. An indirect immunoperoxidase assay successfully detected DSHDV antigens in tissues of infected Pekin ducks as early as 4 hours postinoculation, with no staining in sham-inoculated controls.
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