Key result
A genotype-specific 1-step duplex RT-PCR assay was successfully developed for the simultaneous detection, differentiation, and VP1 sequencing of duck hepatitis A virus types 1 and 3.
Population
Nucleic acids extracted from 2 duck hepatitis A virus reference strains, 9 other infectious viruses and…
Design
Preclinical
Authors
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Offers a tool for DHAV differentiation in poultry outbreaks; leaves open routine use pending field validation.
The developed 1-step duplex RT-PCR assay provides a specific molecular tool for the simultaneous detection, differentiation, and VP1 sequencing of DHAV-1 and DHAV-3 for epidemic investigation.
Wen et al. (2014) studied Duck viral hepatitis (DHAV-1 and DHAV-3). 1-step duplex reverse-transcription PCR assay was evaluated on Detection, differentiation, and VP1 sequencing of DHAV-1 and DHAV-3. A genotype-specific 1-step duplex RT-PCR assay was successfully developed for the simultaneous detection, differentiation, and VP1 sequencing of duck hepatitis A virus types 1 and 3.
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