May 30, 2007Avian Diseases

Development of One-Step Reverse Transcriptase–Polymerase Chain Reaction to Detect Duck Hepatitis Virus Type 1

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Structured PICO

Population

Duckling liver samples infected with 2 reference and 7 Korean field isolates of duck hepatitis virus type 1 (DHV-1), 14 other avian pathogenic viruses and bacteria, and duck/chicken embryos.

Intervention

One-step reverse transcriptase-polymerase chain reaction (RT-PCR) using DHV-1-specific primers against the 3D gene.

Outcome

Detection of DHV-1 (amplification of a 467-bp fragment) and assay sensitivity/specificity.surrogate

A newly developed one-step RT-PCR method provides a rapid, sensitive, and specific tool for detecting duck hepatitis virus type 1.

Discussion

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Implication

Offers sensitive DHV-1 detection for avian research; leaves open field validation before clinical use.

Cite This Study

Kim et al. (2007) studied this question.

synapsesocial.com/papers/69d56f7375589c71d767d8a5 https://doi.org/10.1637/0005-2086(2007)51[540:doortc]2.0.co;2

View Full Paper

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Development of One-Step Reverse Transcriptase–Polymerase Chain Reaction to Detect Duck Hepatitis Virus Type 1

Structured PICO

Discussion

Implication

Cite This Study

Development of One-Step Reverse Transcriptase–Polymerase Chain Reaction to Detect Duck Hepatitis Virus Type 1

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider