Chronic endothelial inflammation in PWH leads to reduced circulating megakaryocyte progenitor cells

Figures

• 

  Figure 1.. Reduced circulating MKP in PWH. Circulating hematogenic progenitor cells were analyzed in PBMCs from PWH (n = 17) and PWOH (n = 16) by flow cytometry. (A) Hematopoietic cell differentiation. CD34+CD38– contains multipotent cells (bold gray text): HSC, MPP, and lymphoid-primed multipotent progenitors (LMPPs). The oligopotent progenitors CD34+CD38+ (black text): common myeloid progenitor (CMP), MEP, and granulocyte-monocyte progenitor (GMP) cells. MKPs originate from the bipotent progenitor MEP (black arrows). HSC-MKPs (stem-cell like) that originate from a subset of HSC-CD41+ (gray arrows). (B) Manual gating strategy. Free platelets (SSClowCD42b+) were excluded from the analysis followed by gating FSC vs SSC, live and single cells. The lineage negative cells (LIN–) were gated by the exclusion of lineage positive cells using a cocktail of mAbs (CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, and CD235a). An additional CD14 mAb was used to eliminated potential contaminant events on the LIN– population (supplemental Figure 1A). The LIN– (gated as LIN–CD14– cells) were analyzed for progenitor marker expression CD34 and CD38: LIN–CD34+CD38– cells (bold gray text) contains…