Key result
Real-time RT-PCR detects ANV and CAstV RNA in up to ~81% of growth-retarded broiler gut samples.
Why the study?
Reliable quantitative detection methods for avian nephritis virus and chicken astrovirus RNAs in chickens were needed to study their prevalence and association with growth retardation.
Population
Broiler chickens including growth-retarded flocks and experimentally infected chicks
Comparison
Two TaqMan-based real-time RT-PCR assays for ANV and CAstV RNA detection
Design
Development and preliminary evaluation of rRT-PCR assays
Follow-up
Up to 35 days
Authors
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High ANV and CAstV loads associate with broiler growth retardation; hypothesis-generating for viral role pending controlled studies.
The developed real-time RT-PCR assays successfully detect and quantify ANV and CAstV RNAs in chicken samples, revealing higher viral loads in growth-retarded flocks.
Smyth et al. (2010) studied Avian nephritis virus and chicken astrovirus infection. Real-time TaqMan RT-PCR assays was evaluated on Detection of ANV and CAstV RNAs. Real-time RT-PCR assays detected high viral RNA levels of ANV and CAstV in 81% and 67% of gut content samples from growth-retarded broiler flocks, respectively.
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