Key result
Pen-based oral fluid collection detected ASFV genome as early as 3-5 days post-infection, suggesting it can supplement traditional individual sampling for rapid surveillance.
Why the study?
Enhanced surveillance and diagnostic testing strategies are critical for early detection and response to African swine fever virus outbreaks in swine populations.
Population
Weaned pigs housed in numbers mimicking industry settings in four independent experiments
Comparison
Pen-based oral fluid sampling vs individual oropharyngeal swabs and blood samples
Design
Four independent experimental animal studies using highly and moderately virulent ASFV strains
Follow-up
Daily oral fluid and swab sampling; blood samples every other day
Authors
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Aggregate sampling may enhance ASF surveillance efficiency in swine; leaves open its integration into North American preparedness protocols.
Goonewardene et al. (2021) studied African swine fever virus (ASFV) infection (n=89). Pen-based oral fluid collection vs. Individual whole blood and oropharyngeal swab sampling was evaluated on Early detection of ASFV genome by real-time PCR. Pen-based oral fluid collection detected ASFV genome as early as 3-5 days post-infection, suggesting it can supplement traditional individual sampling for rapid surveillance.
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