Key result
Real-time RT-PCR detects Influenza A in ~94% of porcine oral fluids, outperforming virus isolation.
Why the study?
The presence of Influenza A virus in porcine oral fluids and the validation of a molecular diagnostic test for these samples were not established.
Population
36 pigs inoculated or mock-inoculated and 910 field porcine oral fluid samples
Comparison
Porcine oral fluid real-time RT-PCR vs nasal swab virus isolation and RT-PCR
Design
Preclinical study with experimental inoculation and field sample testing
Follow-up
Up to 7 days postinfection
Authors
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Supports FLUAV detection in porcine oral fluids by RT-PCR; leaves open routine field surveillance adoption pending further validation.
Absolute Event Rate: 94% vs 0%
Pen-based oral fluids provide an easy and effective collection method for detecting Influenza A virus in pigs using real-time RT-PCR.
Detmer et al. (2011) studied Influenza A virus (n=946). Real-time RT-PCR of porcine oral fluids vs. Virus isolation was evaluated on Detection of FLUAV in oral fluids. Real-time RT-PCR detected Influenza A virus in 94% of oral fluid samples from inoculated pigs, compared to 0% by virus isolation, demonstrating its effectiveness as a rapid diagnostic method.
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