Enzymatic processes fall between the two traditional categories of chemical and biological treatment systems since they involve chemical reactions based on the action of biological catalysts. A variety of enzymes from plants and microorganisms have been reported to play important roles in an array of waste treatment applications. Before the full potential of enzymes may be realized, a number of significant issues remain to be addressed. These include: development of low–cost sources of enzymes in quantities that are required at the industrial scale; demonstration of the feasibility of utilizing the enzymes efficiently under the conditions encountered during wastewater treatment; characterization of reaction products and assessment of their impact on downstream processes or on the environment into which they are released; and identification of methods for the disposal of solid residues, among others.
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Jim A. Nicell (2001) studied this question.