Key result
Perfluorocarbon emulsions maintain ~80% of aerated peak tension and preserve ATP during hypoxia vs K-H solution.
Why the study?
The ability of perfluorocarbon emulsions to prevent anaerobic hypofunction and ATP depletion in myocardium during hypoxia was investigated.
Do perfluorocarbon emulsions prevent anaerobic hypofunction and ATP depletion in isolated guinea pig left atria during hypoxia?
Do perfluorocarbon emulsions prevent anaerobic hypofunction and ATP depletion in isolated guinea pig left atria during hypoxia?
Absolute Event Rate: 80% vs 50%
Perfluorocarbon emulsions delay oxygen desaturation and protect cardiac tissue from ATP depletion and impaired function during hypoxia in a preclinical model.
Perfluorocarbon emulsions may protect hypoxic cardiac tissue in guinea pig models; leaves open translation to clinical ischemia.
The isolated, electrically-driven, guinea pig left atrium was used to study the ability of two perfluorocarbon emulsions to prevent anaerobic hypofunction in the myocardium. A 20% perfluorodecalin (PFD) emulsion maintained peak tension at 80% of aerated levels for more than 20 minutes. Emulsions of perfluorooctylbromide (PFOB) ranging from 25 to 100% produced a similar result, except that the 25% emulsion could not maintain contractions for the entire time period. Peak tension of atria bathed in K-H solution decreased to less than 50% over the same time period. Both 20% PFD and 100% PFOB maintained myocardial ATP levels at pre-hypoxic levels for at least twenty minutes after aeration was terminated. Unaerated atria, bathed in Krebs-Henseleit solution only, exhibited a significant decline in tissue ATP levels at this time. It appears that perfluorocarbon emulsions may delay oxygen desaturation and thereby protect cardiac tissue from ATP depletion and impaired cardiac function associated with hypoxia. This tissue preparation was found to be very useful for determining the efficacy of potential oxygen carriers.
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Eyer et al. (1991) studied Myocardial hypoxia. Perfluorodecalin (PFD) and perfluorooctylbromide (PFOB) emulsions vs. Krebs-Henseleit (K-H) solution only was evaluated on Peak tension during hypoxia. Perfluorocarbon emulsions (20% PFD and 100% PFOB) maintained peak tension at 80% of aerated levels and preserved ATP levels during hypoxia, compared to <50% tension with K-H solution alone.
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