What question did this study set out to answer?

To evaluate the feasibility and effectiveness of an aged osmium tetroxide and potassium ferrocyanide solution as an extracellular tracer in electron microscopy.

January 1, 1982Stain Technology

The Use of Osmium Tetroxide-Potassium Ferrocyanide as an Extracellular Tracer in Electron Microscopy

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Authors

AÁArtur P. ÁguasUniversitas Wijaya Kusuma Surabaya

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Implication

Experimental study demonstrates extracellular tracing in fixed liver tissue using osmium tetroxide-potassium ferrocyanide, highlighting an artifact-reducing method for electron microscopy.

Key Points

To evaluate the feasibility and effectiveness of an aged osmium tetroxide and potassium ferrocyanide solution as an extracellular tracer in electron microscopy.
Treated glutaraldehyde-fixed liver tissue samples with long-term stored solutions of osmium tetroxide and potassium ferrocyanide (Os-Fe).
Assessed tracer distribution across tissue compartments and evaluated cell membrane permeability relative to standard peroxidase and lanthanum techniques.
The Os-Fe solution generated an electron-opaque precipitate that selectively highlighted extracellular spaces without penetrating or diffusing across intact cell membranes.
The technique eliminated tissue preparation artifacts commonly caused by vascular perfusion with peroxidase or immersion in lanthanum, though the prerequisite solution storage period was identified as a drawback.

Cite This Study

Artur P. Águas (1982) studied this question.

synapsesocial.com/papers/6aac81e128dacefbf66e0035 https://doi.org/10.3109/10520298209066530

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Implication

Experimental study demonstrates extracellular tracing in fixed liver tissue using osmium tetroxide-potassium ferrocyanide, highlighting an artifact-reducing method for electron microscopy.

The Use of Osmium Tetroxide-Potassium Ferrocyanide as an Extracellular Tracer in Electron Microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

The Use of Osmium Tetroxide-Potassium Ferrocyanide as an Extracellular Tracer in Electron Microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

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