What question did this study set out to answer?

To determine the biological role of APPL2 in modulating adiponectin and insulin signaling cascades in muscle cells.

August 7, 2009Journal of Biological ChemistryOpen Access

Yin-Yang Regulation of Adiponectin Signaling by APPL Isoforms in Muscle Cells

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Authors

CWChanghua WangBeijing Tongren Hospital XXXiaoban XinJohns Hopkins University RXRuiHua XiangYunnan Normal University

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Implication

In vitro study reveals that APPL2 negatively regulates adiponectin and insulin signaling in muscle cells, indicating an opposing control mechanism between APPL isoforms.

Key Points

To determine the biological role of APPL2 in modulating adiponectin and insulin signaling cascades in muscle cells.
Evaluated the effects of APPL2 overexpression and RNAi-mediated knockdown in C2C12 myotubes on receptor interaction, glucose uptake, and fatty acid oxidation.
Assessed protein interactions between APPL1, APPL2, and adiponectin receptors (AdipoR1 and AdipoR2), including dissociation dynamics upon metformin exposure.
Overexpression of APPL2 inhibited the interaction between APPL1 and AdipoR1, down-regulating adiponectin signaling in C2C12 myotubes.
Knockdown of APPL2 by RNA interference significantly enhanced adiponectin-stimulated glucose uptake and fatty acid oxidation.
APPL2 sequestered APPL1 to suppress both adiponectin and insulin signaling, while treatment with metformin induced APPL1-APPL2 dissociation.

Cite This Study

Wang et al. (2009) studied this question.

synapsesocial.com/papers/6aac85879701df8e5d6e009c https://doi.org/10.1074/jbc.m109.010355

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Implication

In vitro study reveals that APPL2 negatively regulates adiponectin and insulin signaling in muscle cells, indicating an opposing control mechanism between APPL isoforms.

Yin-Yang Regulation of Adiponectin Signaling by APPL Isoforms in Muscle Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Yin-Yang Regulation of Adiponectin Signaling by APPL Isoforms in Muscle Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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