The formation of ω-cyclohexyl-fatty acids from shikimate in an acidophilic thermophilic bacillus. A new biosynthetic pathway

Labelled acetate, phenylalanine and shikimic acid were fed to Bacillus acidocaldarius. A high proportion of the (14)C incorporated from acetate and shikimate was recovered in methyl esters from the cell lipids, but such recovery of (14)C from phenylalanine was low. Only the (14)C from shikimate was selectively incorporated into 11-cyclohexylundecanoate and 13-cyclohexyltridecanoate. Degradation of these cyclohexyl-fatty acids showed that shikimate was incorporated as an intact C(7) unit.