Glycerol-3~phosphate, adenosine triphosphate, coenzyme A, MgC12, and NaF were necessary cofactors for maximal esterifieation of palmitate-l-14C by homogenates of bovine liver and adipose tissue.Dithiothreitol enhanced esterification in liver, but not in adipose tissue homogenates, whereas esterification was stimulated by bovine serum albumin in adipose but not hepatic tissue.Both systems incorporated palmitate, stearate, oleate, and linolenate into glycerides.Linoleate incorporation was scant by either system.Liver homogenates esterified a greater percentage of the fatty acids into phospholipids and monoglycerides than did adipose tissue.Triglycerides accounted for 55% of the product in adipose tissue and 18 to 44% in liver.
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Benson et al. (1971) studied this question.
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