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July 29, 2012AJP Heart and Circulatory PhysiologyOpen Access

TLR4 deficiency reduces cardiac triglycerides and preserves early cardiac function in diabetic mice.

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Why the study?

TLR4 is upregulated in diabetic cardiomyocytes and its role in modulating cardiac fatty acid metabolism and diabetic heart disease pathogenesis was unclear.

Does TLR4 deficiency reduce cardiac lipid accumulation and improve cardiac function in a mouse model of type 1 diabetes?

Population

Nonobese diabetic (NOD) mice and H9C2 cardiomyocytes

Comparison

TLR4-deficient NOD mice vs wild-type NOD mice; TLR4 siRNA vs control in H9C2 cells

Design

Preclinical experimental study

Key result

TLR4 deficiency in nonobese diabetic mice attenuated cardiac triglyceride accumulation and mitigated the impairment of cardiac function in the early stages of diabetes compared with wild-type mice.

Authors

BDBaojun DongDQDake QiLYLong Yang

Discussion

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Member takes

Overview

TLR4 may be a target in diabetic cardiomyopathy models; leaves open translation to human disease or therapy.

Structured PICO

Does TLR4 deficiency reduce cardiac lipid accumulation and improve cardiac function in a mouse model of type 1 diabetes?

P
Population
Nonobese diabetic (NOD) mouse model of type 1 diabetes and H9C2 cardiomyocytes
E
Exposure
TLR4 deficiency (genetic knockout in mice) and TLR4 small interfering RNA (in H9C2 cardiomyocytes)
C
Comparator
Wild-type (WT) NOD mice and control nondiabetic mice
O
Outcome
Cardiac triglyceride accumulation and cardiac function (ejection fraction, fractional shortening, left ventricular developed pressure)surrogate

TLR4 deficiency attenuates cardiac lipid accumulation and preserves cardiac function in a mouse model of type 1 diabetes, suggesting TLR4 as a potential therapeutic target for diabetic cardiomyopathy.

Cite This Study

Dong et al. (2012) studied Type 1 diabetes and diabetic heart disease. TLR4 deficiency vs. Wild-type (WT) NOD mice was evaluated on Cardiac lipid accumulation and cardiac function. TLR4 deficiency in nonobese diabetic mice attenuated cardiac triglyceride accumulation and mitigated the impairment of cardiac function in the early stages of diabetes compared with wild-type mice.

synapsesocial.com/papers/6aac9983df605b4b09f82dffhttps://doi.org/10.1152/ajpheart.00948.2011
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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