Key result
HPTLC and direct densitometry successfully separate and quantify cerebrospinal fluid gangliosides with ~78% recovery.
Why the study?
A method for separation and quantification of complex ganglioside mixtures from clinically available amounts of human cerebrospinal fluid was needed.
Population
Human cerebrospinal fluid samples
Comparison
Ganglioside extraction and quantification by HPTLC and direct densitometry
Design
Analytical method development and validation study
Authors
Loading...
May enable ganglioside analysis from limited CSF volumes; leaves open validation as a neurological biomarker.
The study describes a reliable method for separating and quantifying gangliosides from small volumes of human CSF using HPTLC.
Trbojević-Čepe et al. (1990) studied Normal and neurological diseases (methodological study) (n=10). High-performance thin-layer chromatography (HPTLC) and direct densitometry was evaluated on Method recovery and ganglioside profile. A method using high-performance thin-layer chromatography and direct densitometry successfully separated and quantified gangliosides from 5 ml of human cerebrospinal fluid with a 78% recovery rate.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: