Key result
A new procedure using chloroform/methanol extraction and chromatography successfully purified gangliosides from small volumes of plasma, cerebrospinal fluid, and fibroblasts.
Population
Small volumes of plasma (0.6 mL), cerebrospinal fluid (1 mL) and cultured fibroblasts
Design
Preclinical
Authors
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May enable ganglioside analysis in limited preclinical samples; leaves open human validation for lysosomal disorder diagnostics.
A new procedure for extracting and purifying gangliosides from small biological samples is effective for analyzing lysosomal enzyme activity and ganglioside catabolism.
Leenders et al. (1995) studied Deficiency of lysosomal enzyme activity affecting ganglioside breakdown. Procedure to extract and purify gangliosides was evaluated on Extraction and purification of gangliosides. A new procedure using chloroform/methanol extraction and chromatography successfully purified gangliosides from small volumes of plasma, cerebrospinal fluid, and fibroblasts.
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