What question did this study set out to answer?

To determine whether the insulin-stimulated casein kinase present in liver preparations is identical to the insulin receptor tyrosine kinase.

December 1, 1983European Journal of BiochemistryOpen Access

The insulin‐stimulated receptor kinase is a tyrosine‐specific casein kinase

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Authors

YZYehiel ZickWeizmann Institute of Science RRR W Rees-JonesBrigham and Women's Hospital GGGeorge GrunbergerDiabetes Australia

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Implication

In vitro study demonstrates that insulin-stimulated receptor kinase and casein kinase share identical enzymatic properties in rat liver, indicating a single receptor-bound enzyme.

Key Points

To determine whether the insulin-stimulated casein kinase present in liver preparations is identical to the insulin receptor tyrosine kinase.
Analyzed solubilized, lectin-purified insulin receptor preparations derived from rat liver.
Assayed kinase thermal inactivation at 37 °C and sensitivity to alkylating agents using receptor and casein substrates.
Performed immune coprecipitation using anti-receptor antibodies as well as insulin and anti-insulin antibody complexes.
Insulin selectively increased tyrosine-specific phosphorylation of casein by 2- to 3-fold, mirroring endogenous receptor phosphorylation.
Both receptor and casein kinase activities showed an identical thermal inactivation half-life of 5 minutes at 37 °C and identical responses to alkylating agents.
Both kinase activities quantitatively coprecipitated with anti-receptor or insulin/anti-insulin immune complexes, confirming a single receptor-associated enzyme.

Cite This Study

Zick et al. (1983) studied this question.

synapsesocial.com/papers/6aaca59ed20e92d4d43a80cf https://doi.org/10.1111/j.1432-1033.1983.tb07872.x

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Implication

In vitro study demonstrates that insulin-stimulated receptor kinase and casein kinase share identical enzymatic properties in rat liver, indicating a single receptor-bound enzyme.

The insulin‐stimulated receptor kinase is a tyrosine‐specific casein kinase

Discussion

Member takes

Implication

Key Points

Cite This Study

The insulin‐stimulated receptor kinase is a tyrosine‐specific casein kinase

Discussion

Member takes

Implication

Key Points

Cite This Study

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