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Colloidal chitin1~3) is commonly used as a waterinsoluble substrate for the study ofchitinase (EC 3.3.1.14) and lysozyme (EC 3.2.1.17),because it is chemically identical with native chitin.Colloidal chitin is generally prepared by the method using cone, hydrochloric acid,1} aqueous sulfuric acid,2) or phosphoric acid.3)The
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Hirano et al. (1988) studied this question.
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