Key result
Novel enzymatic dispersion and immunomagnetic bead method successfully isolates pure, VEGF-stimulated human decidual endothelium.
Why the study?
A novel method was needed for the isolation and culture of pure microvascular endothelium from first trimester human decidua to study uterine angiogenesis.
Answers are grounded in this paper and cite their sources.
A novel isolation method successfully yields pure human decidual microvascular endothelium, demonstrating that VEGF specifically stimulates its growth, highlighting its potential role in uterine angiogenesis.
A novel method has been developed for the isolation of microvascular endothelium from first trimester decidua. Enzymatic dispersion and Percoll gradient centrifugation, followed by positive selection using Ulex europaeus coated immunomagnetic beads, yields pure isolates of endothelium. Typical 'cobblestone' monolayers form within 3-5 days; cells express the classical endothelial markers CD31 and Von Willebrand factor and the inducible cell adhesion molecules endothelial-leukocyte adhesion molecule and vascular cell adhesion molecule. Cell growth is dependent on both high concentrations of magnesium ions and human serum. Of the endothelial growth factors examined, only vascular endothelial growth factor stimulated growth, suggesting an important role for this factor in uterine angiogenesis.
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Grimwood et al. (1995) studied First trimester decidua. Isolation and culture method using enzymatic dispersion, Percoll gradient centrifugation, and Ulex europaeus coated immunomagnetic beads was evaluated on Isolation and characterization of microvascular endothelium. A novel method using enzymatic dispersion, Percoll gradient centrifugation, and immunomagnetic beads successfully isolated pure human decidual endothelium whose growth was stimulated by VEGF.
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