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Molecular biological techniques have allowed more questions regarding the structure and function of the nicotinic acetylcholine receptor to be addressed. Toni Claudio describes the progress that has been made in characterizing the receptor-channel complex as regards subunit assembly and expression and explains how cDNA probes can be used to identify receptor subtypes and monitor receptor levels during development. Functional - biochemical and pharmacological - techniques are required to validate the information gained from cloning techniques; pharmacologists are far from being redundant!
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Toni Claudio (1986) studied this question.
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