What question did this study set out to answer?

To evaluate the diagnostic performance of biplate and triplate chromogenic culture media compared to MALDI-TOF mass spectrometry for rapid identification of pathogens causing clinical and subclinical bovine mastitis.

April 30, 2021Journal of Dairy ScienceOpen Access

Evaluation of chromogenic culture media for rapid identification of microorganisms isolated from cows with clinical and subclinical mastitis

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Authors

BGB.M. GranjaUniversidade de São Paulo CFCarlos Eduardo FidelisUniversidade de São Paulo BGBreno Luís Nery GarciaUniversity of Calgary

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Implication

Diagnostic study reveals pathogen-dependent accuracy of rapid chromogenic culture media in dairy cows, indicating potential for targeted on-farm mastitis treatment.

Key Points

To evaluate the diagnostic performance of biplate and triplate chromogenic culture media compared to MALDI-TOF mass spectrometry for rapid identification of pathogens causing clinical and subclinical bovine mastitis.
Diagnostic validation study evaluating biplate and triplate chromogenic media against MALDI-TOF mass spectrometry as the standard reference.
Experiment 1 assessed biplates (CHROMagar) selective for gram-positive and gram-negative bacteria using 476 clinical mastitis (CM) and 660 subclinical mastitis (SCM) milk samples.
Experiment 2 evaluated triplates (Smartcolor2, Onfarm) selective for Streptococcus/Strep-like organisms, Staphylococcus, and gram-negative bacteria using 476 CM and 500 SCM milk samples.
In Experiment 1, biplate sensitivity ranged from 0.56 (95% CI: 0.32–0.81; S. aureus) to 0.90 (95% CI: 0.80–0.99; Strep. uberis) for CM, and from 0.50 (95% CI: 0.15–0.85; E. coli) to 0.94 (95% CI: 0.87–1.00; S. aureus) for SCM; specificity was consistently high across all pathogens (0.94 to 1.00).
In Experiment 2, triplate sensitivity ranged from 0.09 (95% CI: 0.00–0.26; Serratia spp.) to 0.94 (95% CI: 0.85–1.00; Klebsiella and Enterobacter spp.) for CM, and from 0.25 (95% CI: 0.10–0.40; Lactococcus spp.) to 1.00 (Strep. agalactiae/dysgalactiae) for SCM.
Agreement (κ) varied widely across both tests, ranging from 0.07 to 0.85 for CM and 0.18 to 0.88 for SCM, reflecting pathogen-specific diagnostic consistency.

Cite This Study

Granja et al. (2021) studied this question.

synapsesocial.com/papers/6aacfa7c30f0d8c6b4249fdc https://doi.org/10.3168/jds.2020-19513

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Implication

Diagnostic study reveals pathogen-dependent accuracy of rapid chromogenic culture media in dairy cows, indicating potential for targeted on-farm mastitis treatment.

Evaluation of chromogenic culture media for rapid identification of microorganisms isolated from cows with clinical and subclinical mastitis

Discussion

Member takes

Implication

Key Points

Cite This Study

Evaluation of chromogenic culture media for rapid identification of microorganisms isolated from cows with clinical and subclinical mastitis

Discussion

Member takes

Implication

Key Points

Cite This Study

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