What question did this study set out to answer?

To investigate the physiological function of phospholipase Cε in heart development and semilunar valvulogenesis using targeted gene inactivation in mice.

March 1, 2005Molecular and Cellular BiologyOpen Access

Congenital Semilunar Valvulogenesis Defect in Mice Deficient in Phospholipase Cε

View Full Paper

Ask AI

Bookmark

Authors

MTMakoto TadanoHyogo Brain and Heart Center HEHironori EdamatsuJuntendo University SMSusumu MinamisawaJikei University School of Medicine

Discussion

Member takes

Implication

Experimental genetic study demonstrates congenital semilunar valve malformations in phospholipase Cε-deficient mice, suggesting its role in regulating valve remodeling via BMP signaling.

Key Points

To investigate the physiological function of phospholipase Cε in heart development and semilunar valvulogenesis using targeted gene inactivation in mice.
Generated knockout mice harboring a catalytically inactivated phospholipase Cε allele via gene targeting.
Assessed cardiac valve anatomy, valve remodeling, ventricular dimensions, and hemodynamic function in homozygous mutant mice.
Evaluated downstream embryonic signaling cascades by measuring Smad1/5/8 activation at the margins of developing semilunar valve tissues.
Homozygous mutant mice developed congenital malformations in both aortic and pulmonary valves, characterized by marked leaflet thickening, moderate-to-severe regurgitation, mild stenosis, and ventricular dilation.
Valvular remodeling defects arose during late embryonic valvulogenesis, producing a phenotype closely resembling that of epidermal growth factor receptor-attenuated or HB-EGF-deficient mice.
Embryonic semilunar valve tissue margins showed aberrant activation of Smad1/5/8, linking phospholipase Cε deficiency to disrupted suppression of bone morphogenetic protein-mediated cell proliferation.

Cite This Study

Tadano et al. (2005) studied this question.

synapsesocial.com/papers/6aaddca3638974a0e695a7d0 https://doi.org/10.1128/mcb.25.6.2191-2199.2005

View Full Paper

Ask AI

Bookmark

Implication

Experimental genetic study demonstrates congenital semilunar valve malformations in phospholipase Cε-deficient mice, suggesting its role in regulating valve remodeling via BMP signaling.

Congenital Semilunar Valvulogenesis Defect in Mice Deficient in Phospholipase Cε

Discussion

Member takes

Implication

Key Points

Cite This Study

Congenital Semilunar Valvulogenesis Defect in Mice Deficient in Phospholipase Cε

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider