The Effect of Ultra-High Frequency Electromagnetic Radiation on Reparative Osteogenesis and Angiogenesis under Transosseous Osteosynthesis

Background: The use of non-medicinal facilities of correcting processes of bone tissue regeneration for various pathological conditions is one of the most urgent problems of modern medicine. Purpose of the study:To study the efficiency of using low-intensive ultra-high frequency electromagnetic radiation in order to promote reparative osteogenesis and angiogenesis during fracture treatment under transosseous osteosynthesis making a qualitative and quantitative morphological analysis.Materials and methods: Tibial fracture was modeled in the experiment on rats in control and experimental groups with reposition and fixation of the bone fragments.In experimental animals the fracture zone was exposed to low intensity electromagnetic radiation of ultra-high frequency.Exposure simulation was performed in the control group.The operated bones were examined using radiography, light and electronic microscopy, X-ray electron probe microanalysis.Results: Electromagnetic radiation of ultra-high frequency in fracture treatment was proved to stimulate secretory activity and degranulation of mast cells, to produce the increase in microcirculatory bed vascular permeability, endotheliocyte migration phenotype expression, to ensure endovascular endothelial outgrowth, to activate reparative osteogenesis and angiogenesis while fracture reparation becomes one of the primary type and of a short-term duration.The content of bone tissue in the zone of fracture has been demonstrated to exceed the control values by 74%, 89% and 75%, respectively, 7, 14 and 28 days after surgery when exposed to low-intensive electromagnetic radiation of ultra-high frequency.Conclusion: Mast cell secretory activity stimulation and endovascular angiogenesis activation is one of the therapeutic action mechanisms of ultra-high frequency electromagnetic radiation during fracture treatment.