January 30, 1990Philosophical transactions of the Royal Society of London. Series B, Biological sciences
Methylation and expression of the Myo D1 determination gene
Key result
5-azacytidine induces Myo D1 gene demethylation in mouse embryo cells in vitro.
Why the study?
Myo D1 gene methylation status and its role in myogenic determination and differentiation in vitro remain unclear, with potential implications for immortal cell lines and tumors.
Population
Mouse embryo 10T1/2 cells and clonal myogenic or adipogenic derivatives
Comparison
Methylated Myo D1 in 10T1/2 and adipocyte derivatives vs demethylated Myo D1 in myogenic derivatives
Design
Preclinical in vitro study using 5-azacytidine induced differentiation
Authors
Discussion
Loading...
Member takes
Implication
MyoD1 methylation may regulate myogenic differentiation in vitro; leaves open in vivo and tumor roles.
Structured PICO
P
Population
Mouse embryo cells (10T1/2 cells) and their clonal derivatives (myogenic or adipogenic lineages)
I
Intervention
5-azacytidine (demethylating agent)
O
Outcome
Methylation status and expression of the Myo D1 genesurrogate
The Myo D1 gene may be subject to methylation control in vitro, which could have implications for the establishment of immortal cell lines and tumors.
Cite This Study
Jones et al. (1990) studied this question. 5-azacytidine was evaluated on Methylation status of Myo D1 gene. Induction of mouse embryo cells with 5-azacytidine revealed that the Myo D1 gene is demethylated in myogenic derivatives in vitro, though it may not be methylated at CCGG sites in vivo.