Myosin heavy chain expression within the tapered ends of skeletal muscle fibers

BACKGROUND: The pectoralis muscle of the chicken contains fast-twitch glycolytic fibers, which during development undergo a transformation in their myosin heavy chain (MyHC) content from embryonic to a neonatal to an adult isoform (Bandman et al., 1990). Little, however, is known of MyHC expression within the ends of these or other muscle fibers. Here we test the hypothesis that the tapered ends of mature skeletal muscle fibers contain a less mature MyHC isoform than that typically found throughout their lengths. METHODS: We apply an ammoniacal silver histological stain for endomysium and monoclonal antibodies against neonatal and adult MyHCs of chicken pectoralis to transverse serial sections of pectoralis from five mature chickens. The "lesser fiber diameters" of populations of fibers from each bird are also measured. RESULTS: Most (approximately 81.8%) of the small (< 12 microns) and none of the larger (> 20 microns) diameter fibers contain the neonatal MyHC. Following these smaller fibers through serial sections, we show that they are the tapered ends of the larger fibers. Whereas neonatal MyHC is restricted to the tapered fiber ends, adult MyHC is present throughout the entire lengths of all fibers. We also demonstrate acetylcholinesterase (AChE) activity at some of these fiber ends. CONCLUSIONS: We postulate that longitudinal growth of myofibrils in adult muscle is characterized by the sequential expression of MyHC isoforms similar to that observed in rapidly growing muscle and that the presence of the neurotransmitter hydrolase AChE at the tapered fiber ends may be related to the retention of neonatal MyHC.