Key result
Restoring Akt1 in South Asian endothelial cells rescues angiogenesis and vascular repair to European levels.
Why the study?
Reduced vascular repair capacity in South Asian men, who have increased cardiovascular risk, may be linked to impaired function of outgrowth endothelial cells (OEC).
Does restoring Akt1 activity in outgrowth endothelial cells from South Asian men improve vascular reparative potential?
Population
OEC derived from South Asian men and matched European controls
Comparison
Constitutively active Akt1 expression in South Asian OEC vs nonmanipulated European OEC, SA OEC with GFP control, and vehicle
Design
In vitro and in vivo preclinical assays including immunodeficient mouse models of femoral artery injury and hind limb ischemia
Authors
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OEC assays in SA men are hypothesis-generating; leaves open whether targeted manipulation improves vascular repair in high-risk groups.
Does restoring Akt1 activity in outgrowth endothelial cells from South Asian men improve vascular reparative potential?
Reduced signaling via the Akt/eNOS axis is causally linked with impaired OEC-mediated vascular repair in South Asian men, and restoring Akt1 activity rescues this dysfunction.
Cubbon et al. (2014) studied Impaired vascular repair. Expression of constitutively active Akt1 (E17KAkt) vs. Green fluorescent protein control and nonmanipulated European OEC was evaluated on In vitro angiogenesis, re-endothelialization, and perfusion recovery. Restoring Akt1 activity in outgrowth endothelial cells from South Asian men rescued in vitro angiogenesis and in vivo vascular reparative potential to levels comparable with European controls.
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