Down-regulation of lncRNA MALAT1 alleviates vascular lesion and vascular remodeling of rats with hypertension

Figures

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  Figure 1. LncRNA MALAT1 was up-regulated in HTN patients. (A) expression of lncRNA MALAT1 in healthy controls and HTN patients; (B) ROC curve represented for the efficiency of lncRNA MALAT1 expression to the diagnosis of HTN patients. n = 30, data were expressed as mean ± standard deviation.

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  Figure 2. Expression of lncRNA MALAT1 and Notch-1 was elevated in vascular tissues of HTN rats. (A) expression of of lncRNA MALAT1 and Notch-1 in thoracic aorta vascular tissues detected by RT-qPCR; (B) protein band of Notch-1 in thoracic aorta vascular tissues; (C) expression of Notch-1 in thoracic aorta vascular tissues detected by western blot analysis; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.

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  Figure 3. Down-regulated MALAT1 and inhibited Notch-1 decline blood pressure level of rats with HTN. (A) comparison of SBP of rats; (B) comparison of DBP of rats; (C) comparison of MAP of rats; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.

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  Figure 4. Down-regulated MALAT1 and inhibited Notch-1 repress inflammation-related factors expression in rats with HTN. (A) comparison of TNF-α levels in serum of rats; (B) comparison of IL-1β levels in serum of rats; (C) comparison of IL-6 levels in serum of rats; (D) comparison of MCP-1 levels in serum of rats; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.

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  Figure 5. Down-regulated MALAT1 and inhibited Notch-1 reduce endothelial function-related factors expression in rats with HTN. (A) AngII in serum of rats; (B) ET-1 in serum of rats; (C) NO in serum of rats; (D) ICAM-1 in serum of rats; (E) VCAM-1 in serum of rats; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed using Tukey’s post hoc test.

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  Figure 6. Down-regulated MALAT1 and inhibited Notch-1 decline oxidative stress-related factors expression in rats with HTN. (A) comparison of SOD in serum of rats; (B) comparison of MDA in serum of rats; (C) comparison of ROS in serum of rats; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.

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  Figure 7. Down-regulated MALAT1 and inhibited Notch-1 relieve pathological lesion of thoracic aorta. (A) thoracic aorta tissues of rats were observed using HE staining (× 500, scale bar: 20 μm); (B) medial thickness and lumen diameter in thoracic aorta of rats and their ratio; (C) ultrastructure of thoracic aorta of rats were observed by a transmission electron microscopy (× 10000, scale bar: 1 μm). * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.

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  Figure 8. Down-regulated MALAT1 and inhibited Notch-1 alleviate vascular fibrosis. (A) thoracic aorta fibrosis was observed using Masson staining (× 40, scale bar: 250 μm); (B) expression of collagen-I, collagen-III and Fibronectin mRNA in thoracic aorta tissues of rats detected by RT-qPCR; (C) protein bands of collagen-I, collagen-III and Fibronectin in thoracic aorta tissues of rats; (D) expression of collagen-I, collagen-III and Fibronectin in thoracic aorta tissues of rats detected by western blot analysis; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.

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  Figure 9. Down-regulated MALAT1 and inhibited Notch-1 suppress apoptosis of aortic endothelial cells. (A) endothelial cells of thoracic aorta were observed by TUNEL staining (× 200, scale bar: 50 μm); (B) apoptosis index of endothelial cells in thoracic aorta; (C) protein bands of Bax and Bcl-2 of thoracic aorta tissues; (D) expression of Bax and Bcl-2 in thoracic aorta; * P < 0.05 vs the Con group; # P < 0.05 vs the AngII + si-Con group; & P < 0.05 vs the AngII + DMSO group; n = 10, data were expressed as mean ± standard deviation; one-way ANOVA was used for analyzing data, pairwise comparison was analyzed by Tukey’s post hoc test.