Key result
Rapid immunochromatographic and PCR tests for rotavirus detection yield no quantitative diagnostic performance results.
Why the study?
Acute diarrhoea remains a significant public health issue with rotavirus causing severe symptoms especially in children and adults, necessitating evaluation of detection methods.
What is the diagnostic performance of three different routine schemes for the detection of rotaviruses in archived stool samples?
Observational (n=640)
No
What is the diagnostic performance of three different routine schemes for the detection of rotaviruses in archived stool samples?
The study evaluates the diagnostic performance of three different techniques for rotavirus detection in stool samples.
Highlights rotavirus in adult gastroenteritis; leaves open need for validated diagnostic criteria in adults.
Despite the significant medical advances which have taken place in the last decades, acute diarrhoea cases remain a public health issue of major significance, with gastroenteritis agents being associated with severe symptoms in adults and high morbidity in infants and children. Regarding rotaviruses, while children are the predominant victims of rotavirus infection, adults (often caretakers or parents of these children) may experience the same symptoms of fever, vomiting, and non-bloody diarrhoea. Three different routine schemes for the detection of rotaviruses in archived stool samples were evaluated in terms of diagnostic performance. A total of 640 archived stool samples were included in the study. The samples were screened with three different techniques: a commercial rapid immunochromatographic test, a modified in-house conventional one-step reverse-transcription polymerase chain reaction (PCR) screen protocol, and a com-mer-cial one-step real-time PCR kit. Technical aspects and considerations are discussed.
No takes yet. Share an insight, caveat, or question.
Vontas et al. (2019) conducted an observational in Rotavirus infection (n=640). Commercial rapid immunochromatographic test vs. Conventional RT-PCR and real-time PCR was evaluated on Diagnostic performance. A commercial rapid immunochromatographic test, conventional RT-PCR, and real-time PCR were evaluated for rotavirus detection in 640 archived stool samples, with no quantitative results reported in the abstract.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: