Key result
Ridaquick Combi ICT shows ~97% PCR concordance for rotavirus but poor sensitivity for adenovirus.
Why the study?
The study was conducted to retrospectively evaluate the rotavirus and adenovirus immunochromatographic test routinely used in a university hospital virology laboratory.
Does the Ridaquick Rotavirus/Adenovirus Combi ICT accurately detect rotavirus and adenovirus in stool specimens compared to multiplex PCR?
Observational (n=715)
No
Does the Ridaquick Rotavirus/Adenovirus Combi ICT accurately detect rotavirus and adenovirus in stool specimens compared to multiplex PCR?
The Ridaquick ICT is adequate for rapid rotavirus detection but lacks sufficient sensitivity for adenovirus, necessitating PCR-based assays for the latter.
May support ICT for rotavirus screening; leaves open need for PCR confirmation in adenovirus detection.
Immunochromatographic tests (ICT) are diagnostics tools providing rapid results without the need for specialized equipment. Our aim was to evaluate retrospectively the rotavirus and adenovirus ICT routinely used in the virology laboratory serving the University Hospital of Marseille, France. From January 2017 to March 2020, 715 stool specimens from patients were screened using the Ridaquick Rotavirus/Adenovirus Combi ICT (RR/AC ICT) and a commercially available multiplex PCR detection kit. Rotavirus was detected in 9.2% of specimens by PCR and 7.7% of specimens by RR/AC ICT while adenovirus was detected in 8.5% of specimens by PCR and 2.4% of specimens by RR/AC ICT. The RR/AC ICT parameters for rotavirus were 75.8% sensitivity, 99.2% specificity, 90.9% positive predictive value (PPV) and 97.6% negative predictive value (NPV). The RR/AC ICT parameters for adenovirus were 6.6% sensitivity, 98.0% specificity, 23.5% PPV and 91.8% NPV. While the ICT test may be suitable for rotavirus detection, a PCR-based assay is better adapted for adenovirus detection in stools.
No takes yet. Share an insight, caveat, or question.
Simo-Fouda et al. (2021) conducted an observational in Suspected viral gastroenteritis (n=715). Ridaquick Rotavirus/Adenovirus Combi ICT vs. Fast Track Diagnostics Viral Gastroenteritis PCR assay was evaluated on Concordance between ICT and PCR for rotavirus detection (95% CI 95.8-98.3). The Ridaquick Rotavirus/Adenovirus Combi ICT showed acceptable performance for rotavirus detection (75.8% sensitivity, 97.1% concordance with PCR) but poor sensitivity (6.6%) for adenovirus.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: