Key result
Proposed spectrophotometric method accurately measures metronidazole in blood and pharmaceutical samples with high precision.
Why the study?
A sensitive quantitative method for determining metronidazole levels in pharmaceutical preparations and human blood is needed for accurate therapeutic monitoring.
A simple spectrophotometric method was developed for the accurate quantitative estimation of metronidazole in pharmaceutical preparations and human blood, enabling therapeutic monitoring.
Should not yet change metronidazole monitoring; leaves open independent clinical validation studies.
This research investigates a sensitive quantitative estimation of metronidazole (MZOL) in each of its pharmaceutical preparations and its level in human blood with one color reaction and one simple spectrophotometric technique. Metronidazole the antibiotic and antiprotozoal medication is determined by the reduction reaction of MZOL to 2-(2-Methyl-5-amino-1H-imidazole-1-yl) ethanol, followed by coupling with diazotized p-amino benzophenone (PABPh) reagent. The produced color complex is measured at 431 nm. The proposed method is successfully applied for the determination of metronidazole in different dosage forms (tablet, suspension, and intravenous injection) with high precision (RSD% from ±0.011 to ±2.3). as well as it applied for the determination of metronidazole in human blood samples of healthy voluntaries easily and accurately after 4, 6, 12, and 24 hours of oral administration with relative standard deviation not more than ±1.922 which provide the ability for therapeutic monitoring follow up of metronidazole in blood.
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Khalil et al. (2022) studied this question. Spectrophotometric method using diazotized p-amino benzophenone was evaluated on Precision of metronidazole determination (RSD%). A proposed spectrophotometric method successfully determined metronidazole levels in pharmaceutical preparations and human blood samples with high precision (RSD% up to ±2.3).
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