What question did this study set out to answer?

To investigate the TGF-β response and its receptor dynamics during IL-10-mediated activation of human B cells.

September 1, 2019European Cytokine NetworkOpen Access

IL-10 induces TGF-β secretion, TGF-β receptor II upregulation, and IgA secretion in B cells

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Authors

YBYasser BagheriGolestan University FBFateme BabahaQueen's University RFReza FalakIran University of Medical Sciences

Discussion

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Implication

In vitro study demonstrates that IL-10 promotes TGF-β signaling and IgA secretion in activated human B cells, highlighting a cooperative pathway in antibody class switching.

Key Points

To investigate the TGF-β response and its receptor dynamics during IL-10-mediated activation of human B cells.
Negatively purified pan B cells from healthy human donors using magnetic cell separation.
Cultured purified B cells with multimeric CD40 ligand (mCD40L) alone or in combination with IL-10 for 2 and 7 days.
Measured surface TGF-β receptor II and IgA expression by flow cytometry, and quantified TGF-β and IgA secretion via ELISA.
Co-stimulation with mCD40L and IL-10 significantly increased both TGF-β receptor II surface expression and TGF-β secretion at 48 hours compared to mCD40L alone.
IL-10 stimulation significantly enhanced IgA secretion by class-switched plasma cells at day 7 compared to cultures stimulated with mCD40L alone.

Cite This Study

Bagheri et al. (2019) studied this question.

synapsesocial.com/papers/6aae4fff5ccc6a1f121c2424 https://doi.org/10.1684/ecn.2019.0434

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September 1, 2019European Cytokine NetworkOpen Access

IL-10 induces TGF-β secretion, TGF-β receptor II upregulation, and IgA secretion in B cells

View Full Paper

Ask AI

Bookmark

Authors

YBYasser BagheriGolestan University FBFateme BabahaQueen's University RFReza FalakIran University of Medical Sciences

Discussion

Member takes

Implication

In vitro study demonstrates that IL-10 promotes TGF-β signaling and IgA secretion in activated human B cells, highlighting a cooperative pathway in antibody class switching.

Key Points

To investigate the TGF-β response and its receptor dynamics during IL-10-mediated activation of human B cells.
Negatively purified pan B cells from healthy human donors using magnetic cell separation.
Cultured purified B cells with multimeric CD40 ligand (mCD40L) alone or in combination with IL-10 for 2 and 7 days.
Measured surface TGF-β receptor II and IgA expression by flow cytometry, and quantified TGF-β and IgA secretion via ELISA.
Co-stimulation with mCD40L and IL-10 significantly increased both TGF-β receptor II surface expression and TGF-β secretion at 48 hours compared to mCD40L alone.
IL-10 stimulation significantly enhanced IgA secretion by class-switched plasma cells at day 7 compared to cultures stimulated with mCD40L alone.

Cite This Study

Bagheri et al. (2019) studied this question.

synapsesocial.com/papers/6aae4fff5ccc6a1f121c2424 https://doi.org/10.1684/ecn.2019.0434

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